[Objective] The ecological service value and interval compensation relationship of Shaanxi section of Weihe River basin were studied in order to provide theoretical guidance for promoting the coordinated development of regional ecological economy and ecological environment construction. [Methods] Based on the land use data of Shaanxi section of Weihe River basin, the ecosystem service value (ESV) and its spatial distribution and spatio-temporal evolution were studied by using land use dynamics, equivalent factor method and barycenter model method, and the compensation relationship was analyzed with priority. [Results] ① Under the influence of the project of returning farmland to forest and the rapid progress of urbanization, the reduction of cultivated land and the increase of construction land in spatial quantity were obvious, among which the single dynamic degree of construction land reached 5.94%, which was most active; The grassland, water body and other land use were more intense in the degree of evolution. ② The ESV in Shaanxi section of the Weihe River basin decreased from 56.207 billion yuan to 51.621 billion yuan, a decrease of more than 8%. Among them, the value of functional services such as water supply and hydrological regulation decreased, and the increase of forest land increased the value of functional services such as climate regulation and soil conservation; The spatial distribution of ESV showed a pattern of “low in the middle and high around”; The shift of the center of gravity and the standard deviation ellipse of the construction land reflect that it has the trend of moving in the northeast direction and the regional scope continues to expand. [Conclusion] Zhouzhi County and Taibai County were the priority compensation areas. Lantian County, Long County and Feng County were the secondary compensation areas, and compensation should be obtained in time to ensure ecological quality. Yanta District and 11 other regions were compensation areas, and compensation funds should be provided to maintain the overall ecological construction.