[Objective] The spatiotemporal changes of ecosystem service value (ESV) during the development and construction of Meizhou Island (a ‘cultural’, ‘pilgrimage’, and ‘tourism’ island) during 2000—2020 were analyzed in order to provide a scientific reference for the formulation of land use planning and ecological construction for the tourism area of the island. [Methods] The global 30 m fine land cover dynamic monitoring data covering Meizhou Island from 1985 to 2020 were used as the data source, and ArcGIS technology was used to extract the land use data for 2000, 2005, 2010, 2015, and 2020 in the study area. The land use changes on Meizhou Island during the recent 20 years was revealed through land use dynamics, land use transfer matrix, and ecological contribution rate. The value equivalent method was used to estimate ESV for Meizhou Island. [Results] ① From 2000—2020, the land use types on Meizhou Island were mainly construction land and cultivated land. Land use area changes were mainly manifested as increases in construction land and wetland, and decreases in cultivated land and forest land. The land use transfer was reflected in the mutual flow among forest land-cultivated land-construction land-water area, and the encroachment of construction land on cultivated land and forest land; ② During the study period, the ESV of Meizhou Island decreased by 12.526 9 million yuan, of which forest land and cultivated land were the main contributing factors of ESV for Meizhou Island. The increases of wetland and water area were the main influencing factors of the increase of ESV; ③ The values of each individual ecosystem service function followed the order of regulation service＞support service＞supply service＞cultural service; ④ The spatial distribution of ESV showed a pattern of ‘lower in the middle and higher on the periphery’, and the distribution of high value areas overlapped highly with that of forest land. Low ESV areas were concentrated in the construction land. [Conclusion] Forest and cultivated land were the main contributors of ESV on Meizhou Island. Regulation service contributed the most to ESV. Therefore, during the development and construction process, we should increase the utilization efficiency of construction land and strengthen the protection of forest land and cultivated land.