[Objective] The function evolution and ecological effects of productional-living-ecological space in scenic spots were explored, in order to provide reference and decision-making basis for the spatial function optimization and ecological environment protection of productional-living-ecological space of scenic spots in Guizhou Province. [Methods] The study was conducted for Guizhou Province as an example, and used land use status data from 2009 and 2021 to construct the productional-living-ecological space. The data were analyzed by combining the productional-living-ecological space spatial transfer matrix, ecological environmental quality index, and ecological contribution rate methods. [Results] ① From 2009 to 2021, scenic areas in Guizhou Province were mainly characterized by ecological space, with an increase in living and ecological space and a decrease in production space. ② During the research period, the overall ecological environmental quality of scenic areas in Guizhou Province increased from 0.59 to 0.61, showing a spatial pattern of “higher in the east and lower in the west”, with a significant increase in high-quality and higher quality areas, which was mainly related to a series of ecological protection and restoration engineering programs implemented in Guizhou Province in recent years, as well as carrying out work such as resource protection and forestry reform. ③ The ecological environmental quality of scenic areas in Guizhou Province has significantly improved from 2009 to 2021, mainly due to the conversion of agricultural production land and grassland ecological land to forest ecological land. [Conclusion] The ecological environment quality of scenic spots in Guizhou Province has been significantly improved. In the follow-up planning and management, it is suggested to strictly control the growth of construction land and strengthen the ecological environment advantages of scenic spots.