[Objective] To provide theoretical reference for realizing sustainable development and ecological civilization construction in the Weihe River Basin.[Method] Build remote sensing ecological index (RSEI) based on GEE platform to dynamically evaluate the change of ecological environment quality in the Weihe River Basin from 2000 to 2020, and explore the impact of climate, vegetation, terrain, soil and socio-economic factors on ecological environment quality based on the optimal parameter geographic detector model. The results show that: [Results] (1) from 2000 to 2020, the ecological environment quality in the region increases; the ecological environment quality grade is mainly poor and poor, accounting for more than 60% of the total area.; In 20 years, the ecological environment quality grade area increased more for the middle, good and excellent ecological environment quality.(2) The ecological environment quality grade has a spatial distribution pattern of "high in the south and low in the north, high in the east and low in the west". The poor ecological environment area has the largest area.73 43% of the Weihe River Basin is in the ecological environment quality increase area of 12.09×104km2;during the study period, the area where the ecological environment quality increases and the reduced area keeps increasing, and the overall ecological environment quality of the research area develops in a better direction. The area of excellent ecological environment quality is still inclined in the overall distribution along the southeast-northwest direction.(3) Rainfall, elevation and soil organic carbon content are the main factors affecting the ecological environment quality of the Weihe River Basin;.[Conclusion] In the process of improving the ecological environment quality of the Weihe River Basin, the distribution law of slope should be fully considered to optimize the governance scheme.