[Objective] In order to provide scientific support for environmental evolution in Guizhou karst area and countermeasures for adapting to and mitigating climate change in the region changing regulations of temperature and precipitation in Yangtze River basin in Guizhou Province in recent 57 years were analyzed.[Methods] Based on data of monthly average temperature and precipitation of 12 meteorological stations in Yangtze River basin of Guizhou Province from 1960 to 2016, and applying methods of cumulative anomaly, 5 a moving average, Mann-Kendall mutation test and regression analysis, climate characteristics and change were analyzed.[Results] The results showed that temperature trend in the basin had increased in general, with an increasing ratio of 0.14℃/10 a. There was a slight fluctuated downward trend for precipitation, with a decrease ratio of 12.94 mm/10 a. A temperature inflection occurred in 1997, there was a great difference in temperature before and after the inflection point, but no obvious change was found in precipitation. Resulted from the different effect of longitude and altitude, temperature and precipitation is different obviously in east-westward direction. In all water systems, temperature was mainly in a rising trend. Precipitation showed a M shaped fluctuation with slight downward trend. Correlation analysis showed that precipitation decreased with increase of temperature, especially the change of precipitation in Wujiang River had very high sensibility with temperature.[Conclusion] In general, the basin is consistent with the trend of climate change throughout Guizhou Province and even in southwest China as well, and is evolving toward the directions of warming and drying. In order to slow down this trend, vegetation ratio should be increased so as to improve regional microclimate.